Electrophoresis Reagents SDS removal kit
AE-1390 SDS-eliminant
SDS removal reagent
AE-1390 SDS-eliminant is the reagent for removing SDS (Sodium dodecyl sulfate) from samples including SDS by 2 steps.
AE-1390 SDS-eliminant is the reagent for removing SDS (Sodium dodecyl sulfate) from samples including SDS by 2 steps. At first, Solution A removes dodecyl sulfate ion to precipitate it by insoluble pellet. The SDS also bound to proteins directly, however, could not be removed by Solution A. Second, the non-ionic surfactant including Solution B makes mixed micelle with SDS. This step additionally removes the SDS direct-binding to the proteins. SDS-eliminant is extremely useful method in sample isolation, fractionation and purification.
AE-1390 SDS-eliminant
Purpose and Application
- Removal of SDS from the sample solution
- Removal of SDS from the proteins isolated SDS-PAGE
- Removal of SDS from the cell or tissue extracts
- Preparation of Mass Spectrometry, electrophoresis and chromatography
- Reactivation of enzymes* denatured by SDS (* not all enzymes)
Product Summary
Solution A removes dodecyl sulfate ion to precipitate it by insoluble pellet. The SDS also bound to proteins directly, however, could not be removed by Solution A. Second, the non-ionic surfactant including Solution B makes mixed micelle with SDS. This step additionally removes the SDS direct-binding to the proteins.
Features
- Ready to Use
- Over 95% of the ionized SDS is removed briefly
- The recovery success rate of proteins is high
- Available for small amount samples (less than 1mL)
Specifications
| Product number | AE-1390 | |
|---|---|---|
| Product name | SDS-eliminant | |
| Components | Solution A | Solution B |
| Major constituent | KCl, Tris | non-ionic surfactant, Tris |
| Package | 2mL solution / a tube | 2mL solution / a tube |
| Max sample volume | 40mL | 10mL |
| Storage | Cold storage in light-blocking bag, one year guarantee | |
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